| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186144 |
| Chromosome: | chromosome 4 |
| Location: | 2815139 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224150 | (1 of 1) 2.7.1.30 - Glycerol kinase / Glycerokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTGACACCTGGTCGCGGCACCGCAAG |
| Internal bar code: | GCCGACTAGATACGCCCGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 98.8636 |
| LEAP-Seq n confirming: | 2088 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATCTCAAGTGGGTCGT |
| Suggested primer 2: | CAAAGTGACCTCATCTGCGA |