| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186147 |
| Chromosome: | chromosome 7 |
| Location: | 5065558 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347550 | (1 of 1) PTHR23389:SF6 - ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCATAGGCCGTTGCCTTGTTGCCGTACA |
| Internal bar code: | CTCAGTAATAATCCCAGATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 99.2553 |
| LEAP-Seq n confirming: | 5331 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTCGCGTTGCAGATCAC |
| Suggested primer 2: | GGATCTGGATTTGGACGTTG |