Insertion junction: LMJ.RY0402.186148_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.186148
Chromosome:chromosome 16
Location:4504177
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g667350 CAV6 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAAGGGTGGGGGCGCCGTCGCCGGTAGG
Internal bar code:TTGTGGTTCGGGTCGAGCTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:448
LEAP-Seq percent confirming:96.6524
LEAP-Seq n confirming:2252
LEAP-Seq n nonconfirming:78
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCATTTCCCAGAAAGGAACA
Suggested primer 2:AGGAAGTCACCAACCAGGTG