| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186161 |
| Chromosome: | chromosome_14 |
| Location: | 920970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613700 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGATGAATGATCGGCGCGGATAGCTGGTA |
| Internal bar code: | GGGAATGACCGAGGATCTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 97.6858 |
| LEAP-Seq n confirming: | 15027 |
| LEAP-Seq n nonconfirming: | 356 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTCTGGTCTGCGTTTG |
| Suggested primer 2: | GCGTTGTAGGCTCCAGAAAG |