| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186205 |
| Chromosome: | chromosome 8 |
| Location: | 1933354 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367350 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTGCTCCTGCTGCTGCTCCCGTGCGT |
| Internal bar code: | AGTCCCGTAAGCGAGCACGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 98.0969 |
| LEAP-Seq n confirming: | 2835 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCTGTGTGTGCTTGTGT |
| Suggested primer 2: | TTGTTGCAGCAGGCAATTAG |