| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186212 |
| Chromosome: | chromosome 12 |
| Location: | 5238659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528200 | (1 of 1) K10742 - DNA replication ATP-dependent helicase Dna2 (DNA2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGACACACGAGGCACGGGCACCCGATG |
| Internal bar code: | CGTCTAACACTCTGGCGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 0.303797 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1969 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGACGAGTGCGTTGGAG |
| Suggested primer 2: | GTTCTTACCCTCCTGGGCTC |