| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186212 |
| Chromosome: | chromosome 12 |
| Location: | 5238824 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528200 | (1 of 1) K10742 - DNA replication ATP-dependent helicase Dna2 (DNA2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGTGCAGAGCAGAGAGGCGGCGGCGGG |
| Internal bar code: | ACGTGCTTGCGCGGACAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 99.6689 |
| LEAP-Seq n confirming: | 301 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTTACCCTCCTGGGCTC |
| Suggested primer 2: | GCACCAGTGACAGCAGGAT |