| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186223 |
| Chromosome: | chromosome 8 |
| Location: | 2143936 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369150 | (1 of 1) PTHR10566:SF50 - PROTEIN LACT-3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCACGTGACGCCAAGCCGACGTGACGCC |
| Internal bar code: | GCGTAGCAGGTTATGGATCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 99.9036 |
| LEAP-Seq n confirming: | 9324 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAACGGAGTGCTCAAAG |
| Suggested primer 2: | ACCCTCAAAAACACACTCCG |