| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186270 |
| Chromosome: | chromosome 12 |
| Location: | 7015141 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559750 | PRL2 | (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACAACCAGCGCGCATTTGCCATTCACA |
| Internal bar code: | AGGCGCACATTTCCCAGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 98.5329 |
| LEAP-Seq n confirming: | 2082 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACCTCGCTAGTCTCAAC |
| Suggested primer 2: | CAGTTCATAGCCAGCGTCAA |