| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186273 |
| Chromosome: | scaffold_24 |
| Location: | 121154 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre24.g755997 | PHC18,FAP150 | Cell wall protein pherophorin-C18 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAGTCGGGCACGACGCTGACCGACCTCTC |
| Internal bar code: | AGCGCCTACAGCGAGGGTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 99.7026 |
| LEAP-Seq n confirming: | 1341 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAATTTTCGTGAGTGCTGC |
| Suggested primer 2: | AGCGGGTACACCTTCATCAC |