Insertion junction: LMJ.RY0402.186290_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.186290
Chromosome:chromosome 3
Location:6599442
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g196400 EPC1 (1 of 1) K11322 - enhancer of polycomb-like protein (EPC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGATGAGCCACGCACCTTGCTGCCAGT
Internal bar code:GTGAAGACGAAAGCCGAGTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTGGAGGCAAGGTTTGT
Suggested primer 2:CCGGGTGATTGTTCCTTCTA