| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186327 |
| Chromosome: | chromosome 8 |
| Location: | 4322438 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381450 | OPR35 | OctotricoPeptide Repeat protein 35 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACAGAGCGAGGAGGAGAGCGTGGGCGA |
| Internal bar code: | GAAACGAGGTGCCCTTCCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 95.7647 |
| LEAP-Seq n confirming: | 3256 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCCTACGGGACTTTAC |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |