| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186360 |
| Chromosome: | chromosome 7 |
| Location: | 672373 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317300 | ROC78,MAPKKKK1,SIK1 | (1 of 1) PTHR24361:SF268 - SERINE/THREONINE-PROTEIN KINASE 3; Mitogen Activated Protein Kinase Kinase Kinase Kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCTGCCGAGCTAGTTCCAGCCTGCAAG |
| Internal bar code: | GGCATTCGCTTTAGACTTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 99.4399 |
| LEAP-Seq n confirming: | 8167 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCTGTGACTGGGATCA |
| Suggested primer 2: | TGAGCCCTGGCTGAGTAGAT |