Insertion junction: LMJ.RY0402.186365_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.186365
Chromosome:chromosome 2
Location:7151991
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g146500 DSK1,DYRK1 Dual-specificity tyrosine regulated protein kinase; (1 of 1) K18669 - dual specificity tyrosine-phosphorylation-regulated kinase 2/3/4 (DYRK2_3_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAGCGCCGGCAACCCGCGACTGCAGCCC
Internal bar code:GTCGACTAAGGATGGCCGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:394
LEAP-Seq percent confirming:94.9035
LEAP-Seq n confirming:6443
LEAP-Seq n nonconfirming:346
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCCTGCGATACATGAAG
Suggested primer 2:GTTGCTGTTGCTGTTGCTGT