| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186383 |
| Chromosome: | scaffold 18 |
| Location: | 234547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749847 | DLD1 | Dihydrolipoyl dehydrogenase; (1 of 1) PTHR22912:SF151 - DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAGACAGGGGTGGCCTGACCAGTGACC |
| Internal bar code: | CCCCGGGTACCGACCTCGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGGTAGACCATCTTGCG |
| Suggested primer 2: | GCTAAGAACATCCTGCTGGC |