| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186407 |
| Chromosome: | chromosome 12 |
| Location: | 5371248 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529700 | FAP401 | Putative Flagellar Associated Protein 401 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTATCTACAGCTCAAATCTACACGCGTG |
| Internal bar code: | TCTCCGCTGGCGACGCCCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 98.9375 |
| LEAP-Seq n confirming: | 1583 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAAGTGACCACTGCTCG |
| Suggested primer 2: | GCATTTCTGTCGTCGCACTA |