| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186427 |
| Chromosome: | chromosome 5 |
| Location: | 1922487 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241633 | (1 of 2) IPR006626//IPR011050//IPR012334 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Pectin lyase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGGGCTGCCGCGTGCACGACTGCGC |
| Internal bar code: | GCCAGATCGTGGCCTACGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 67.1715 |
| LEAP-Seq n confirming: | 1774 |
| LEAP-Seq n nonconfirming: | 867 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTAGGCAGCAGGCGTAG |
| Suggested primer 2: | TCCAATGGCCAAGCCTATAC |