| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186439 |
| Chromosome: | chromosome 16 |
| Location: | 6012095 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676450 | CPLD39 | Conserved in the Plant Lineage and Diatoms | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTTTTGGCCATGTTTGCAGGGAGTGAA |
| Internal bar code: | AACGGGTGCGTAACACCCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAAGGTGAGGTGGCGTTG |
| Suggested primer 2: | CTAGACTCAGCAAGGGTGCC |