| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186446 |
| Chromosome: | chromosome 6 |
| Location: | 796214 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254200 | SRL1C,SRLc | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGTGTTGACGACCGTGGCAGGCGCCG |
| Internal bar code: | CGCGGACAACCTGGTTGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 5.58778 |
| LEAP-Seq n confirming: | 289 |
| LEAP-Seq n nonconfirming: | 4883 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGCAGCGCATTTGATTTG |
| Suggested primer 2: | CTGGTACATGAAAGAGCGCA |