| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186448 |
| Chromosome: | chromosome 8 |
| Location: | 4263169 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAACTGCTTATTCTACGTTCAAAGATCC |
| Internal bar code: | AATGTTTCGAGGGTATAATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.836 |
| LEAP-Seq n confirming: | 4870 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGTCCTCATGTCAGCCA |
| Suggested primer 2: | AGGCCACGTGCTTTTAGGTA |