| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186451 |
| Chromosome: | chromosome 17 |
| Location: | 1583124 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707600 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAGTGAGGCCGGTCCTGACACATACAG |
| Internal bar code: | CGCTAGGGCTCAGAGGGCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 98.9726 |
| LEAP-Seq n confirming: | 1734 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTCGGACTGTGAATTT |
| Suggested primer 2: | CTGCATTGATGATGTCTGGG |