| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186461 |
| Chromosome: | chromosome 1 |
| Location: | 7012252 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050616 | 3'UTR | ||
| Cre01.g050624 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATGCAGGCATCGAAGGTTTGCTTGGTG |
| Internal bar code: | GACAGCCGTGGGCGAAACGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 99.2467 |
| LEAP-Seq n confirming: | 10804 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGAGGAGGCTGTTGTC |
| Suggested primer 2: | ACAGACTGCTGCTGTGGTTG |