| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186469 |
| Chromosome: | chromosome 13 |
| Location: | 1554009 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573000 | E2FR1 | (1 of 2) PTHR13271//PTHR13271:SF44 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE SMALL SUBUNIT N-METHYLTRANSFERASE I-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAACGCATGCAATTCACTCACTCTGGTA |
| Internal bar code: | GGGCACGCGCCGTCATCCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 91.4373 |
| LEAP-Seq n confirming: | 299 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCATCCACAGGGTTGTCT |
| Suggested primer 2: | TCTACAAGTGAAACGCACGG |