Insertion junction: LMJ.RY0402.186478_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.186478
Chromosome:chromosome 5
Location:744705
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g248650 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGGCCGGCGGCGGGGCCGGCGGCGGC
Internal bar code:TACCCAAACAAAGTCATTGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:419
LEAP-Seq percent confirming:99.8484
LEAP-Seq n confirming:1976
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACGC
Suggested primer 2:AGTCTCTGCCGTTAGCCTCA