| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186480 |
| Chromosome: | chromosome 8 |
| Location: | 1281197 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363500 | (1 of 2) 4.2.1.134 - Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAAGGAATCGTCGCCGGGGGGGGAGGC |
| Internal bar code: | ATGCAGCAGATCGCAAGCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 96.9466 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTCCCTTCCCTTGTGTG |
| Suggested primer 2: | ATCCTGGACACCTTACACGC |