| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186482 |
| Chromosome: | chromosome 6 |
| Location: | 121054 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249950 | (1 of 3) K00889 - 1-phosphatidylinositol-4-phosphate 5-kinase (PIP5K) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGTGCGGCACGGACTGGGCCGGCAGG |
| Internal bar code: | TGGGACTGTAATCATGCGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 99.8182 |
| LEAP-Seq n confirming: | 1098 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGCTTGAGATGCAGTGA |
| Suggested primer 2: | GAGCGGACAGGTCAGAGAAC |