Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.186497 |
Chromosome: | chromosome_17 |
Location: | 4284186 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g730750 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATCCAGCAGCCGCACCGCAGCAGCCGTTC |
Internal bar code: | ACAGGCCCGTTAGAGAGGGGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1059 |
LEAP-Seq percent confirming: | 92.842 |
LEAP-Seq n confirming: | 2633 |
LEAP-Seq n nonconfirming: | 203 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTCGAACACTCACGGGTTT |
Suggested primer 2: | ACATACGCTTACCCAGCCAC |