| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186531 |
| Chromosome: | chromosome_3 |
| Location: | 9186805 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g212865 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCTAGTGTGCATGCGGGGAGCGGGCGTGT |
| Internal bar code: | CGTTACTGAGCACGCTTGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 98.8837 |
| LEAP-Seq n confirming: | 2746 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGTGGTCATGAAGCTGG |
| Suggested primer 2: | ACCATTCCCAACTTGCTGTC |