| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186548 |
| Chromosome: | chromosome 2 |
| Location: | 6098696 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113200 | GLN1,NSG18 | (1 of 2) PTHR20852:SF50 - GLUTAMINE SYNTHETASE CYTOSOLIC ISOZYME 1-1-RELATED; Glutamine synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAAACCCGCCAAGCAATCCAACGAGGGC |
| Internal bar code: | AATGGCGGGACATTGTCATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.7666 |
| LEAP-Seq n confirming: | 1710 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCTGTCAGCACTTTGTC |
| Suggested primer 2: | CGTCTGACTCGTCTCCATGA |