Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.186548 |
Chromosome: | chromosome_2 |
Location: | 6098696 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g113200 | GLN1,NSG18 | Glutamine synthetase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGTAAACCCGCCAAGCAATCCAACGAGGGC |
Internal bar code: | AATGGCGGGACATTGTCATACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 306 |
LEAP-Seq percent confirming: | 99.7666 |
LEAP-Seq n confirming: | 1710 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGCCTGTCAGCACTTTGTC |
Suggested primer 2: | CGTCTGACTCGTCTCCATGA |