| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186552 |
| Chromosome: | chromosome 16 |
| Location: | 2093464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657700 | (1 of 2) PF13661 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTGTAAGGTCAGAAGAGCGGGACCTGG |
| Internal bar code: | AGCTATGACTCTAGCCGCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 98.5733 |
| LEAP-Seq n confirming: | 4560 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGATGTGGTACATAGGCG |
| Suggested primer 2: | CAACATTGCGTGTCTGTGTG |