| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186573 |
| Chromosome: | chromosome 15 |
| Location: | 1133786 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641000 | NUP50 | Nucleoporin 50; (1 of 2) PF00638 - RanBP1 domain (Ran_BP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGTGGCAATGAAAGTGAAACCGCCCGT |
| Internal bar code: | AGCGGGGGCGTAAGGGGTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 99.7693 |
| LEAP-Seq n confirming: | 2595 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATCCATAGACCGTCGTG |
| Suggested primer 2: | GTCCTGCTCTTGGTCCTGAG |