Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.186574 |
Chromosome: | chromosome_2 |
Location: | 5524278 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g108450 | FAP280 | Flagellar Associated Protein, transcriptional coactivator-like | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGAGGGAAGAAGGACAGGTGAACCCAGGGA |
Internal bar code: | CTGAAAGGTGCGCCGACGGTAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 956 |
LEAP-Seq percent confirming: | 93.9891 |
LEAP-Seq n confirming: | 172 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCATATGCATGATGCTCCGT |
Suggested primer 2: | AGAAATGTGGGTCGTTCTGG |