| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186580 |
| Chromosome: | chromosome 3 |
| Location: | 3852282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170800 | (1 of 1) PTHR12231//PTHR12231:SF138 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACGGGTACACCGCAGTAATAAAGGGAG |
| Internal bar code: | AGGTGGTATCTCCTTCTCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 98.8546 |
| LEAP-Seq n confirming: | 1122 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATACGGTACCGCACGACT |
| Suggested primer 2: | TTTCCAAGGTGTTTGGCTTC |