| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186655 |
| Chromosome: | chromosome 13 |
| Location: | 4680181 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTTTGAGCACGCACGCACGCTAACCTC |
| Internal bar code: | AGACGGGCCAAAAAGTAGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 93 |
| LEAP-Seq percent confirming: | 98.7541 |
| LEAP-Seq n confirming: | 1823 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAACCCGGTATGTTGTGG |
| Suggested primer 2: | CCGGTGAAGATATTGCGTTT |