Insertion junction: LMJ.RY0402.186683_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.186683
Chromosome:chromosome_8
Location:4661475
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre08.g383600 SRR21 Scavenger receptor cysteine rich (SRCR) protein antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGCTTTCGCTGCATGTGCCACGCGCTTCT
Internal bar code:TATCGTGGAGAATGTTAGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:446
LEAP-Seq percent confirming:98.0132
LEAP-Seq n confirming:148
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGCACGCTCATGTTAGG
Suggested primer 2:GGCAAAACACTTGCTCGAAT