Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.186683 |
Chromosome: | chromosome_12 |
Location: | 1212154 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g489550 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGCCCGCCCCGACCACAGGCGCCGCTCC |
Internal bar code: | CGGCCACCTTGCGTAACGGTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 501 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 130 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATCATGCTTACCAGTTGCG |
Suggested primer 2: | ATGCAGACGACGACACTGAC |