| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186692 |
| Chromosome: | chromosome 4 |
| Location: | 2765826 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGGGTGCTGCCCATCTTCAGAATACAC |
| Internal bar code: | TAGGGGCGTAATGACCTGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 89.5926 |
| LEAP-Seq n confirming: | 3409 |
| LEAP-Seq n nonconfirming: | 396 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTGTCACTGGAGGTAGG |
| Suggested primer 2: | ATCTACTGGGGACTACCGCC |