| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186705 |
| Chromosome: | chromosome 8 |
| Location: | 3220309 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373500 | (1 of 1) K10308 - F-box protein 31 (FBXO31) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACTCTCCTGCTCCTGTCAATTCTCATG |
| Internal bar code: | CCGCCGCAGCATATAGGCCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.8138 |
| LEAP-Seq n confirming: | 1072 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGCTGCTGACTAGGCT |
| Suggested primer 2: | ACTTACGACCCCGTGTTACG |