| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186766 |
| Chromosome: | chromosome 9 |
| Location: | 2310551 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392251 | (1 of 28) 2.7.7.6 - DNA-directed RNA polymerase / RNA polymerase III | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGCCGGGGCCGCGTCGAGCTTTCTCT |
| Internal bar code: | GGCACACGTCATAGATTATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 998 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCTCCAGAACCACTTGC |
| Suggested primer 2: | GCGGATACGTGAGTTACGGT |