| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186791 |
| Chromosome: | chromosome 6 |
| Location: | 2273195 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266850 | (1 of 1) PTHR10071:SF199 - GATA-BINDING PROTEIN 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATTGCTAGCACCCGCGTAGGCGCCCAG |
| Internal bar code: | ATCTGACTGAATACTGGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.2878 |
| LEAP-Seq n confirming: | 697 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGCAGCATAACTTGCAG |
| Suggested primer 2: | GTTCAAAAGCCTAAGCGCAC |