| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186798 |
| Chromosome: | chromosome 13 |
| Location: | 5099423 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607350 | CAH7 | (1 of 2) PTHR11002:SF13 - BETA CARBONIC ANHYDRASE 1; Carbonic anhydrase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGTCTAACCCTTGAGGGGTGCACAGCG |
| Internal bar code: | AAACGCCTGGAGGCGGACATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 99.0426 |
| LEAP-Seq n confirming: | 931 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGTCATGCCAGTAGTGT |
| Suggested primer 2: | TAGCTTTGCGCCTAGATGGT |