| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186804 |
| Chromosome: | chromosome 4 |
| Location: | 2132248 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219600 | (1 of 9) IPR000210//IPR011042//IPR011333 - BTB/POZ domain // Six-bladed beta-propeller, TolB-like // POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTCTCCGCTAGCATCTAAACGCGCCGT |
| Internal bar code: | GAGCGGACTTACGTGGGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.4575 |
| LEAP-Seq n confirming: | 1100 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTGTCGGTCTCCATTC |
| Suggested primer 2: | GCATCATACAACGCATACCG |