Insertion junction: LMJ.RY0402.186828_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.186828
Chromosome:chromosome 13
Location:3735157
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589400 PRL6 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCAAATGATGATCTTTGCCGCTGTACA
Internal bar code:CCGTCGCGCCCTGTGGGGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:789
LEAP-Seq percent confirming:99.3575
LEAP-Seq n confirming:6031
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGACCTTGGTCACCACCT
Suggested primer 2:ATGAAACACAGGTTGGCCTC