| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186838 |
| Chromosome: | chromosome 2 |
| Location: | 6144680 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113500 | (1 of 1) IPR001322//IPR014867 - Lamin Tail Domain // Spore coat protein CotH | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGACAGACTGCACCACAAACTCCGATC |
| Internal bar code: | GCCGGCTGTTCGTCAGCTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 99.7211 |
| LEAP-Seq n confirming: | 1430 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTCAGGGCTTCAGGAGGA |
| Suggested primer 2: | GCAGAGTGTGCATGTGTGTG |