| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186849 |
| Chromosome: | chromosome 12 |
| Location: | 3181078 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499250 | PDE10 | (1 of 4) K13293 - cAMP-specific phosphodiesterase 4 (PDE4); 3'%252C5'-cyclic-nucleotide phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGAGTTGACAGAGGCGGGGTGGGACTG |
| Internal bar code: | TACGGTCAAGGGCAGAAATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 35 |
| LEAP-Seq percent confirming: | 89.3162 |
| LEAP-Seq n confirming: | 209 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGGCTTCTGGAGGTAGA |
| Suggested primer 2: | GTGACGTGCAGTGAAGCTGT |