| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186850 |
| Chromosome: | chromosome 5 |
| Location: | 1594018 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243452 | (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCGGGCTCAGCAGTCCCGTGTTGGCA |
| Internal bar code: | TGGCTAGTGTGCGTCATATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 99.5455 |
| LEAP-Seq n confirming: | 219 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTACACATGCCACTGGA |
| Suggested primer 2: | GTCGCGTCCTTCTCTAGGTG |