| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186855 |
| Chromosome: | chromosome 5 |
| Location: | 1202182 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245350 | (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCGACGGCGGCAATGAGCAGGAGGAGC |
| Internal bar code: | ATGAGGACCACAAATTCACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 81.0606 |
| LEAP-Seq n confirming: | 2354 |
| LEAP-Seq n nonconfirming: | 550 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATTGCGTGAAACCTACGG |
| Suggested primer 2: | TGGCTACGCCTTCACTTCTT |