| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186862 |
| Chromosome: | chromosome 12 |
| Location: | 4357422 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520173 | (1 of 1) PTHR10887//PTHR10887:SF340 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGTGAGCAAAGGCCCTACGACAAGTAT |
| Internal bar code: | GTCTTCGGTTAAGGTAAGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 95.587 |
| LEAP-Seq n confirming: | 1148 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGACCTGGCAGTAATGGG |
| Suggested primer 2: | TCAGCAGCAGCATACGGTAG |