| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186863 |
| Chromosome: | chromosome 16 |
| Location: | 7400340 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685613 | (1 of 1) PF02689//PF05970 - Helicase (Herpes_Helicase) // PIF1-like helicase (PIF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCTGTTGTGAGCCAGGTGTGGAAGGCA |
| Internal bar code: | TAGGGTCCAGCATCTCGCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 99.4994 |
| LEAP-Seq n confirming: | 795 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGAGCTCAGTTCAGCAG |
| Suggested primer 2: | CCACCAAGACTGTCCACCTT |