Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.186869 |
Chromosome: | chromosome_6 |
Location: | 1732171 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g261750 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCATAACAGAGGCTGCAACCGGTGGGCGGG |
Internal bar code: | GGCAGGCCCGGCCGCGTGTATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 290 |
LEAP-Seq percent confirming: | 93.5528 |
LEAP-Seq n confirming: | 2728 |
LEAP-Seq n nonconfirming: | 188 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCGAGGGATAATGATCGTG |
Suggested primer 2: | GACGGAAGTTGAAGAGACGC |